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CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Updated: Feb 12, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

High-throughput genetic screens using CRISPR-Cas9 system.

Jiyeon Kweon¹, Yongsub Kim²

¹Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea.

Archives of Pharmacal Research

|April 12, 2018

Summary

This summary is machine-generated.

CRISPR-Cas9 gene editing technology enables precise genome engineering. CRISPR-based pooled libraries accelerate high-throughput screening for regulatory elements, aiding drug discovery and biomedical research.

Keywords:

CRISPR–Cas9 system Genome engineering High-throughput screening Pooled CRISPR screens

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

The CRISPR-Cas9 system offers versatile genome engineering capabilities.
Its programmability allows for gene disruption and regulatory element perturbation.
CRISPR-based pooled libraries are key for high-throughput genetic screening.

Purpose of the Study:

To review recent advancements in CRISPR-Cas9 technology.
To highlight its application in high-throughput genetic screening.
To discuss its potential in drug target discovery and current challenges.

Main Methods:

Review of CRISPR-Cas9 technology and applications.
Focus on CRISPR-based pooled libraries for screening.
Analysis of CRISPR's role in drug discovery and biomedical research.

Main Results:

CRISPR-Cas9 facilitates diverse gene manipulation techniques.
Pooled libraries enable efficient screening of functional genomic elements.
The technology shows significant promise for identifying novel drug targets.

Conclusions:

CRISPR-Cas9 is a transformative tool in genome engineering and screening.
Its application in high-throughput screening accelerates biological discovery.
Further development is needed to overcome challenges in biomedical research.