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Updated: Feb 12, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay
Mian Sahib Zar1,2,3,4,5, Ahmad Ali Shahid4, Muhammad Saqib Shahzad4
1Center for Synthetic Biology Engineering Research (CSynBER), Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xuevuan Avenue, Shenzhen University Town, Shenzhen, China.
Abstract:
This study introduces a newly developed in-house SNaPshot single-base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with a certain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases.
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