Related Experiment Video

Updated: Feb 12, 2026

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

9.1K

Microfluidic enrichment of plasma cells improves treatment of multiple myeloma

Yunjing Zeng1,2, Li Gao1,2, Xiaoqing Luo2

  • 1Division of Periodontology, Diagnostic Sciences & Dental Hygiene, Division of Biomedical Sciences, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.

Molecular Oncology
|April 12, 2018
PubMed

Insights

A novel microfluidic device enhances multiple myeloma (MM) diagnosis by improving the detection of critical cytogenetic alterations in plasma cells (PCs). This method increases diagnostic accuracy and aids in better patient risk stratification and treatment selection.

Area of Science:

  • Hematology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Cytogenetic alterations are crucial for multiple myeloma (MM) risk stratification and therapy selection.
  • Conventional pathology assays on bone marrow samples can yield false-negatives due to rare or unevenly distributed MM cells.
  • Accurate detection of genetic abnormalities in plasma cells (PCs) is essential for effective MM management.

Purpose of the Study:

  • To develop and evaluate a microfluidic device for CD45 depletion and PC enrichment to improve cytogenetic analysis in MM.
  • To enhance the sensitivity and accuracy of detecting cytogenetic alterations in MM plasma cells.
  • To assess the clinical utility of microfluidic-assisted CD45 depletion for MM diagnosis.

Main Methods:

  • A microfluidic device was used for CD45+ leukocyte depletion and size-based plasma cell (PC) selection from bone marrow samples of 48 MM patients.
  • Enriched PCs were analyzed using flow cytometry and fluorescence in situ hybridization (FISH).
  • Results were compared to conventional flow cytometry and FISH analysis without enrichment.

Main Results:

  • The microfluidic method (MF-CD45-TACs) significantly increased the percentage of CD38+/CD138+ cells (37.7% vs. 10.3%, P < 0.001).
  • Detection rates for IgH rearrangement, del(13q14), del(17p), and 1q21 gains were significantly increased post-enrichment (e.g., IgH rearrangement: 56.3%, P < 0.001).
  • The microfluidic assay demonstrated significantly higher detection rates for key cytogenetic alterations compared to the classic method.

Conclusions:

  • Microfluidic-assisted CD45 depletion and PC enrichment improve the detection of cytogenetic alterations in multiple myeloma.
  • This technology offers a more precise diagnostic tool for MM, potentially leading to improved clinical outcomes.
  • The MF-CD45-TACs assay represents a significant advancement in the pathological analysis of multiple myeloma.
Keywords:
microfluidicmyelomarisk stratification

More Related Videos

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma07:53

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

Published on: April 15, 2022

4.8K
Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis10:04

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

Published on: May 1, 2015

13.5K

Related Concept Videos

Multiple Allele Traits01:49

Multiple Allele Traits

The Concept of Multiple Allelism
38.2K
Protein Buffers in Blood Plasma and Cells01:20

Protein Buffers in Blood Plasma and Cells

The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
4.0K
Multiple Regression01:25

Multiple Regression

Multiple regression assesses a linear relationship between one response or dependent variable and two or more independent variables. It has many practical applications.
Farmers can use multiple regression to determine the crop yield based on more than one factor, such as water availability, fertilizer, soil properties, etc. Here, the crop yield is the response or dependent variable as it depends on the other independent variables. The analysis requires the construction of a scatter plot...
4.0K
Composition of Blood Plasma01:24

Composition of Blood Plasma

Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins,...
8.8K
Improving Translational Accuracy02:07

Improving Translational Accuracy

3.7K
Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.0K