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Updated: Feb 12, 2026

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
Published on: November 18, 2011
Localization Microscopy of Actin Cytoskeleton in Human Platelets
Sandra Mayr1, Fabian Hauser2, Anja Peterbauer3
1School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria, Garnisonstr. 21, 4020 Linz, Austria. sandra.mayr@fh-linz.at.
Abstract:
Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm.
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