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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
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Clinicopathological analysis of polyploid diffuse large B-cell lymphoma.
Joji Shimono1,2, Hiroaki Miyoshi1, Junichi Kiyasu3
1Department of Pathology, Kurume University, School of Medicine, Kurume, Japan.
Plos One
|April 12, 2018
Summary
Polyploid chromosomal abnormalities in diffuse large B-cell lymphoma (DLBCL) are linked to poorer overall survival. This finding identifies polyploidy as a potential new prognostic factor for DLBCL patients.
Area of Science:
- Oncology
- Cytogenetics
- Hematology
Background:
- Polyploid chromosomes, having more than two sets of homologous chromosomes, are associated with poor prognosis in various cancers.
- Diffuse large B-cell lymphoma (DLBCL) is a common non-Hodgkin lymphoma with variable outcomes.
- The prognostic significance of polyploid chromosomal abnormalities in DLBCL remains unstudied.
Purpose of the Study:
- To investigate the clinicopathological features and prognostic impact of polyploid chromosomal abnormalities in DLBCL.
- To compare outcomes between DLBCL patients with and without polyploid chromosomal abnormalities.
Main Methods:
- Cytogenetic analysis using G-banding to identify polyploidy (near-tetraploid or greater chromosome number).
- Flow cytometric immunophenotyping and immunohistochemical staining were performed.
- Statistical comparison of clinicopathological features and survival analysis between polyploid DLBCL (51 cases) and control DLBCL (53 cases).
Main Results:
- Polyploid DLBCL exhibited significantly worse overall survival (OS) compared to non-polyploid DLBCL (p = 0.04).
- This survival disadvantage persisted even in patients treated with R-CHOP regimens (p = 0.02).
- Multivariate analysis confirmed polyploid chromosomal abnormalities as an independent prognostic factor for OS.
Conclusions:
- Polyploid chromosomal abnormalities, detected by G-banding, represent a novel poor prognostic factor in DLBCL.
- Identification of polyploidy may aid in risk stratification and treatment decisions for DLBCL patients.
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