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Here, we present a protocol for fabricating channeled agar pads using PDMS molds created from vinyl records. The channels enable users to finely orient Caenorhabditis elegans to improve imaging contrast and facilitate the comparison of structures. These capabilities are particularly useful in neuroregeneration studies.

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This article provides a step-by-step guide to correcting presbyopia with a monocular bi-aspheric ablation...

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data 08:12

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This protocol is a beginner's entryway into processing, fitting, and interpreting transient absorption spectra. The focus of this protocol is the preparation of datasets, and fitting using both single wavelength kinetics and global lifetime analysis. Challenges associated with transient absorption data and its fitting are discussed.

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Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software,...

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