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Updated: Feb 12, 2026
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
Published on: May 2, 2017
MiR-30a-5p confers cisplatin resistance by regulating IGF1R expression in melanoma cells
Yuxia Li1, Jie Zhang2, Yajing Liu1
1Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, No. 1120 Lianhua Road, Futian District, Shenzhen, Guangdong province, China.
Background:
Melanoma is notoriously resistant to all current modalities of cancer therapies including chemotherapy. In recent years, microRNAs (miRNAs) have emerged as molecular regulators in the development and progression of melanoma. However, the relationship between microRNA and chemo-resistance of melanoma is little known. In present study, we aimed to investigate the miRNAs related to cisplatin-resistance in melanoma cells.
Methods:
After cisplatin (DDP) resistant melanoma cells (M8/DDP and SK-Mel-19/DDP) were established in-vitro, high-throughput screening of differentially expressed miRNAs between resistant cells and parental cells were performed.
Results:
It was found that a cancer-related miRNA, miR-30a-5p, was highly over-expressed in resistant cells. Transfection of miR-30a-5p mimic or inhibitor could alter the sensitivity of melanoma cells to cisplatin. Next, we showed that Insulin Like Growth Factor 1 Receptor (IGF1R) gene turned out to be a direct target of miR-30a-5p. Knockdown of IGF1R in melanoma cells could not only reduce the sensitivity to cisplatin but also lead to cell cycle arrest by regulating phosphorylation of Serine-Threonine Protein Kinase (P-AKT (Ser473)) and Tumor Protein P53 (P53).
Conclusion:
Taken together, our study demonstrated that miR-30a-5p could influence chemo-resistance by targeting IGF1R gene in melanoma cells, which might provide a potential target for the therapy of chemo-resistant melanoma cells.
Insights
Melanoma chemo-resistance is linked to elevated miR-30a-5p levels. This microRNA targets Insulin Like Growth Factor 1 Receptor (IGF1R), influencing cisplatin sensitivity and cell cycle progression in melanoma cells.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Melanoma exhibits significant resistance to conventional cancer therapies.
- MicroRNAs (miRNAs) play a role in melanoma development and progression.
- The specific role of miRNAs in melanoma chemo-resistance remains largely unknown.
Purpose of the Study:
- To investigate microRNAs associated with cisplatin resistance in melanoma cells.
- To elucidate the molecular mechanisms underlying chemo-resistance in melanoma.
Main Methods:
- Establishment of cisplatin-resistant melanoma cell lines (M8/DDP and SK-Mel-19/DDP).
- High-throughput screening to identify differentially expressed miRNAs.
- In-vitro functional assays including miRNA mimic/inhibitor transfections and gene knockdown.
Main Results:
- miR-30a-5p was significantly over-expressed in cisplatin-resistant melanoma cells.
- Modulating miR-30a-5p levels altered melanoma cell sensitivity to cisplatin.
- Insulin Like Growth Factor 1 Receptor (IGF1R) was identified as a direct target of miR-30a-5p.
- IGF1R knockdown reduced cisplatin sensitivity and induced cell cycle arrest via AKT and p53 pathways.
Conclusions:
- miR-30a-5p influences chemo-resistance in melanoma by targeting IGF1R.
- This miR-30a-5p/IGF1R axis presents a potential therapeutic target for chemo-resistant melanoma.
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