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Related Concept Videos

Polyprotic Acids03:38

Polyprotic Acids

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Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:
32.1K
Lewis Acids and Bases02:33

Lewis Acids and Bases

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In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
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Ions as Acids and Bases02:54

Ions as Acids and Bases

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Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
26.6K
Acids, Bases and Neutralization Reactions03:26

Acids, Bases and Neutralization Reactions

63.9K
An acid-base reaction is one in which a hydrogen ion, H+, is transferred from one chemical species to another. Such reactions are of central importance to numerous natural and technological processes, ranging from the chemical transformations within cells or lakes and oceans to the industrial-scale production of fertilizers, pharmaceuticals, and other substances essential to the society.
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Bronsted-Lowry Acids and Bases02:58

Bronsted-Lowry Acids and Bases

105.8K
The acid-base reaction class has been studied for quite some time. In 1680, Robert Boyle reported traits of acid solutions that included their ability to dissolve many substances, to change the colors of certain natural dyes, and to lose these traits after coming in contact with alkali (base) solutions. In the eighteenth century, it was recognized that acids have a sour taste, react with limestone to liberate a gaseous substance (now known to be CO2), and interact with alkalis to form neutral...
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Acid-Base Titration Curves02:23

Acid-Base Titration Curves

141.8K
A titration curve is a plot of some solution property versus the amount of added titrant. For acid-base titrations, solution pH is a useful property to monitor because it varies predictably with the solution composition and, therefore, may be used to monitor the titration’s progress and detect its endpoint. Acid-base titration can be performed with a strong acid and a strong base, a strong acid and a weak base, or a strong base and a weak acid.
For a titration carried out for 25.00 mL of...
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Related Experiment Video

Updated: Feb 12, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

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An Ascorbic Acid Oxidase-based Sensing Platform for Stereoselective Interaction with Ascorbic Acid and Isoascorbic

Jiao Ma1, Juanjuan Xu2, Chengcheng Yang1

  • 1Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|April 13, 2018
PubMed
Summary
This summary is machine-generated.

A novel enzyme-based nanohybrid sensor effectively distinguishes ascorbic acid (AA) from isoascorbic acid (IAA). This biosensor utilizes immobilized ascorbic acid oxidase (AO) on gold nanoparticles and graphene for sensitive and rapid stereoselective recognition.

Keywords:
Stereoselective interactionascorbic acidascorbic acid oxidaseisoascorbic acidnanohybrid

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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

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A Polyaniline-based Sensor of Nucleic Acids

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Related Experiment Videos

Last Updated: Feb 12, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

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A Polyaniline-based Sensor of Nucleic Acids
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A Polyaniline-based Sensor of Nucleic Acids

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Area of Science:

  • Electrochemistry
  • Nanomaterials Science
  • Biotechnology

Background:

  • Ascorbic acid (AA) and isoascorbic acid (IAA) are isomers with distinct biological and industrial relevance.
  • Developing selective and sensitive methods for their differentiation is crucial.
  • Enzyme-based biosensors offer high specificity for molecular recognition.

Purpose of the Study:

  • To fabricate a novel enzyme-based nanohybrid material for stereoselective detection of AA and IAA.
  • To utilize ascorbic acid oxidase (AO) as a stereoselective recognition element.
  • To enhance signal amplification and sensor performance using a gold nanoparticle and reduced graphene oxide nanohybrid.

Main Methods:

  • Fabrication of a glassy carbon electrode modified with flower-like electrodeposited gold nanoparticles (dpAu) and reduced graphene oxide (rGO).
  • Immobilization of ascorbic acid oxidase (AO) onto the dpAu/rGO modified electrode.
  • Electrochemical detection of ascorbic acid (AA) and isoascorbic acid (IAA) using the fabricated nanohybrid sensor.

Main Results:

  • The nanohybrid material facilitated high loading of AO and provided signal amplification.
  • The sensor demonstrated clear peak current differences between AA and IAA, enabling stereoselective recognition.
  • A good linear response for both AA and IAA was observed in the concentration range of 1.0 × 10-4 - 5.0 × 10-3 M.

Conclusions:

  • The developed enzyme-based nanohybrid sensor offers a simple, rapid, and effective method for distinguishing AA and IAA.
  • The combination of AO, dpAu, and rGO provides a synergistic effect for enhanced sensing performance.
  • This approach shows significant promise for the practical identification and quantification of AA and IAA.