Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrostatic interactions in the cellular dynamics of the interferon-receptor complex.

G Uze1, M T Bandu, P Eid

  • 1Laboratoire d'Oncologie Virale, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.

European Journal of Biochemistry
|February 1, 1988
PubMed
Summary

Interferon (IFN) binding to its receptor is pH-dependent, with specific amino acid changes influencing this interaction. This pH titration is crucial for understanding interferon

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial intelligence-based prediction of neurocardiovascular risk score from retinal swept-source optical coherence tomography-angiography.

Scientific reports·2024
Same author

[Ultra-widefield imaging of fundus albipunctatus discovered in a 30-year-old woman seen for night blindness].

Journal francais d'ophtalmologie·2023
Same author

Women's reproductive span: a systematic scoping review.

Human reproduction open·2022
Same author

Erratum: "Rovibronic analysis of the Jahn-Teller effect in CH<sub>2</sub>D<sub>2</sub> <sup>+</sup> at low energies" [J. Chem. Phys. 131, 024309 (2009)].

The Journal of chemical physics·2021
Same author

Tropospheric Emissions: Monitoring of Pollution (TEMPO).

Journal of quantitative spectroscopy & radiative transfer·2020
Same author

Structure and dynamics of the radical cation of ethane arising from the Jahn-Teller and pseudo-Jahn-Teller effects.

Physical chemistry chemical physics : PCCP·2017

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Interferons (IFNs) are critical for immune response.
  • Understanding IFN-receptor interactions is key to their therapeutic applications.
  • Recombinant alpha-interferons (IFN-alpha) exhibit varying binding affinities.

Purpose of the Study:

  • To investigate the pH-dependent binding of human recombinant alpha-interferons to their receptors.
  • To identify the molecular determinants responsible for pH-sensitive binding.
  • To elucidate the role of electrostatic interactions in IFN-receptor complex stability.

Main Methods:

  • Utilized membrane preparations from Daudi cells expressing interferon receptors.
  • Examined binding of IFN-alpha A, IFN-alpha B, and IFN-alpha D across a pH range of 6-9.

Related Experiment Videos

  • Constructed hybrid IFNs to map the binding titration region.
  • Analyzed amino acid sequences of key interferon variants.

    • Main Results:

    • IFN-alpha A and IFN-alpha D binding showed significant pH titration, increasing from pH 6 to 9.
    • IFN-alpha B binding was largely unaffected by pH changes in the 6-9 range.
    • A specific region (amino acids 61-92) was identified as responsible for the pH titration.
    • Key amino acid residues Lys-84 and Tyr-90 were implicated in the pH-dependent electrostatic interaction.

    Conclusions:

    • The pH titration of IFN-alpha binding is mediated by electrostatic interactions within the IFN-receptor complex.
    • Specific amino acid residues, particularly Lys-84 and Tyr-90, dictate this pH sensitivity.
    • This pH-dependent binding mechanism may influence the antiproliferative effects of interferons.