Proof of concept: liver splitting during normothermic machine perfusion
Related Experiment Video
Updated: Feb 12, 2026

Normothermic Ex Vivo Liver Machine Perfusion in Mouse
Published on: September 25, 2023
Barney T F Stephenson1, Glenn K Bonney2, Richard W Laing1
1NIHR Birmingham Liver Biomedical Research Unit and Centre for Liver Research, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Introduction:
Despite utilizing extended criteria donors, there remains a shortage of livers for transplantation. No data exists on splitting donor livers with concurrent NMP-L.
Methods:
A liver recovered from a donor after circulatory death was subjected to NMP-L using a red cell based fluid. During NMP-L, a 'classical' left lateral + right trisegmentectomy split was performed using an integrated bipolar/ultrasonic device. After splitting, blood flow was confirmed using Doppler ultrasound in each lobe.
Results:
Prior to splitting, flow rates were maintained physiologically. Lactate decreased from 13.9 to 3.0 mmol/L. Lactate before and after splitting were similar in the hepatic arteries, portal veins and IVC. Doppler ultrasound demonstrated arterial and venous waveforms in both lobes after splitting.
Conclusions:
'Classical' liver splitting during NMP-L is feasible, maintaining viability of both lobes. Establishing this procedure may attenuate cold ischaemic injury, allow pre-implantation monitoring of both grafts and facilitate logistics of transplanting two grafts.
Related Concept Videos
Social Proof
Self-Concept
Infancy and Emerging Recognition
During infancy, self-concept is virtually nonexistent. Babies do not distinguish themselves as separate entities and often mistake their...
Concepts and Prototypes
The brain organizes this information using concepts, which are mental categories grouping linguistic data,...
Machines
A free-body diagram of the...
Formula Mass and Mole Concepts of Compounds
Concept of Pressure at a Point
In a fluid at rest, pressure acts equally in...

