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Updated: Feb 12, 2026

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Published on: March 17, 2015
From isolation of human ovarian follicles to the artificial ovary: tips and tricks
Maria C Chiti1, Jacques Donnez2, Christiani A Amorim1
1Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
Abstract:
Cryopreservation and transplantation of ovarian tissue has proved to restore both endocrine and reproductive functions in cancer patients. However, women with a high risk of ovarian involvement cannot undergo ovarian tissue transplantation due to possible recurrence of the disease. Therefore, different teams worldwide are now working on the development of experimental strategies, like the transplantable artificial ovary (TAO), with the noble goal to offer to these patients hope for future conception. This article discusses some essential points for the creation of a TAO and reviews the latest progress made.
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