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CD3/Ti gamma A: a functional gamma-receptor complex expressed on human peripheral lymphocytes
F Faure1, S Jitsukawa, F Triebel
1Département de Biologie Clinique, Institut Gustave-Roussy, Villejuif, France.
Journal of Immunology (Baltimore, Md. : 1950)
|March 1, 1988
Summary
This study shows that a specific antibody (anti-Ti gamma A) identifies a T-cell subset using gamma-chains as functional receptors. These gamma-chain T-cell receptors mediate crucial immune responses like proliferation and cytotoxicity.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- A novel monoclonal antibody, anti-Ti gamma A, was developed.
- This antibody immunoprecipitates a CD3-associated disulfide-linked gamma-glycoprotein.
- It identifies a subset of adult peripheral lymphocytes (CD2+ CD3+ TCR alpha/beta- CD4- NKH1-).
Purpose of the Study:
- To investigate if gamma-chains on these adult lymphocytes function as T-cell receptors (TCRs).
- To explore the roles of IL-2-dependent proliferation and non-MHC-restricted cytotoxicity in TCR gamma+ cells.
Main Methods:
- Utilized anti-Ti gamma A monoclonal antibody (mAb) to analyze lymphocyte subsets.
- Investigated resting and activated Ti gamma A+ lymphocytes.
- Assessed natural killer (NK) activity, proliferation, and IL-2 secretion.
Main Results:
- Activated Ti gamma A+ lymphocytes exhibited strong cytotoxic function against NK target cell lines.
- Cytotoxic function was modulated by anti-Ti gamma A mAb.
- Resting Ti gamma A+ lymphocytes proliferated and secreted IL-2 upon stimulation with anti-Ti gamma A beads and IL-2.
Conclusions:
- A significant subset of circulating CD3+ TCR alpha/beta- lymphocytes utilizes T-cell gamma rearranging gene products as functional receptor structures.
- Gamma-chain T-cell receptors are involved in immune responses including proliferation and cytotoxicity.