Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.8K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.8K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.4K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.4K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

979
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
979
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

2.9K
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
2.9K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

273
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
273

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same author

Sequencing Ablation and Systemic Therapy in Colorectal Liver Oligometastases: An Upfront versus Delayed Approach Nationwide Analysis.

Radiology·2026
Same author

HA thermostability mutations S84F, G167N, and D168N potentiate H9N2 virus transmission in a warming environment.

Emerging microbes & infections·2026
Same author

RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation.

iScience·2026
Same author

In Situ Synchronized SERS-SEIRAS Unveils Cation-Regulated Interfacial Water and Intermediates in the Oxygen Reduction Reaction.

Angewandte Chemie (International ed. in English)·2026
Same author

Spatial imaging of water oxidation on single-particle catalysts.

Nature nanotechnology·2026
Same journal

Incorporation of Engineered Cu<sup>0</sup>/Cu<sup>+</sup> Interfaces in Metal-Organic Frameworks for Boosting CO<sub>2</sub> Hydrogenation to Methanol.

Angewandte Chemie (International ed. in English)·2026
Same journal

Planar Chiral Carbazole-Naphthalene Bisimide Hetero-Cyclophane for Circularly Polarized Delayed Fluorescence.

Angewandte Chemie (International ed. in English)·2026
Same journal

Charge-Transfer Exciton Flows: Red Luminescent Zn<sub>8</sub>D<sub>14</sub>A<sub>4</sub> Nanotubes.

Angewandte Chemie (International ed. in English)·2026
Same journal

Au(III) Complexes as Pyroptosis Inducers by Targeting Mitochondrial DNA for Tumor Immunity.

Angewandte Chemie (International ed. in English)·2026
Same journal

Suppressing Interfacial-Accelerated Degradation in Perovskite Solar Cells via Supramolecular Co-Assembly.

Angewandte Chemie (International ed. in English)·2026
Same journal

Isolation and Reactivity of a Stannabismuthene.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Related Experiment Video

Updated: Feb 12, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

3.0K

Shell-Isolated Tip-Enhanced Raman and Fluorescence Spectroscopy.

Ya-Ping Huang1, Sheng-Chao Huang1, Xiang-Jie Wang2

  • 1MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces,iChEM, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

Angewandte Chemie (International Ed. in English)
|April 13, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed shell-isolated tips for tip-enhanced Raman and fluorescence spectroscopies. These novel tips overcome contamination and quenching issues, enabling simultaneous signal acquisition for advanced surface analysis.

Keywords:
Raman spectroscopyatomic layer depositionfluorescenceshell-isolated tiptip-enhanced Raman spectroscopy

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.3K
Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

3.5K

Related Experiment Videos

Last Updated: Feb 12, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

3.0K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.3K
Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

3.5K

Area of Science:

  • Surface Science
  • Spectroscopy
  • Nanotechnology

Background:

  • Tip-enhanced Raman spectroscopy (TERS) offers high spatial resolution but suffers from tip contamination and artifacts.
  • Tip-enhanced fluorescence is challenging due to metal tip-induced quenching.

Purpose of the Study:

  • To develop novel shell-isolated tips for overcoming limitations in TERS and tip-enhanced fluorescence.
  • To enable simultaneous acquisition of Raman and fluorescence signals with enhanced resolution.

Main Methods:

  • Fabrication of ultrathin shell-isolated tips using atomic layer deposition.
  • Application of these tips in tip-enhanced Raman spectroscopy (TERS) and tip-enhanced fluorescence.
  • Simultaneous acquisition and manipulation of Raman and fluorescence signals by adjusting shell thickness.

Main Results:

  • Shell-isolated tips demonstrated significant electromagnetic field enhancement in TERS.
  • Effective suppression of tip contamination and fluorescence quenching was achieved.
  • Tip-enhanced fluorescence enhancement factors up to 1.7×10^3 were observed, consistent with simulations.
  • Simultaneous TERS and fluorescence measurements were successfully performed, with tunable signal intensities.

Conclusions:

  • Shell-isolated tips provide a robust platform for ultrahigh-resolution surface analysis using plasmon-enhanced spectroscopies.
  • This approach significantly advances TERS and tip-enhanced fluorescence applications, particularly in solution.
  • The ability to manipulate relative signal intensities by shell thickness offers new possibilities for spectroscopic analysis.