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Updated: Feb 12, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Published on: August 12, 2019
Development and Synthesis of DNA-Encoded Benzimidazole Library
Yun Ding1, Jing Chai1, Paolo A Centrella1
1GlaxoSmithKline , Platform Technology & Science , 200 Cambridgepark Drive , Cambridge , Massachusetts 02140 , United States.
Abstract:
Encoded library technology (ELT) is an effective approach to the discovery of novel small-molecule ligands for biological targets. A key factor for the success of the technology is the chemical diversity of the libraries. Here we report the development of DNA-conjugated benzimidazoles. Using 4-fluoro-3-nitrobenzoic acid as a key synthon, we synthesized a 320 million-member DNA-encoded benzimidazole library using Fmoc-protected amino acids, amines and aldehydes as diversity elements. Affinity selection of the library led to the discovery of a novel, potent and specific antagonist of the NK3 receptor.
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