Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:46

Epigenetic Regulation

33.9K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.9K
GTPases and their Regulation02:14

GTPases and their Regulation

9.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.9K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.6K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.6K
Positive Regulator Molecules01:45

Positive Regulator Molecules

136.5K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polyphosphate synthesis is essential for phosphate and ATP homeostasis during nutrient upshift.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Efficacy and safety of oral DFD-29 versus doxycycline in rosacea: A systematic review and meta-analysis.

Clinical and experimental dermatology·2026
Same author

Stress testing reveals selective vulnerabilities in protein homeostasis.

Cell reports·2026
Same author

Ruxolitinib cream improves outcomes in atopic dermatitis: An updated systematic review and meta-analysis.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026
Same author

Imiquimod 5% for lentigo maligna of the head, neck and face: A systematic review and meta-analysis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2025
Same author

Stress testing reveals selective vulnerabilities in protein homeostasis.

bioRxiv : the preprint server for biology·2025
Same journal

Lactate as a Chemical Modification on Proteins and Metabolites.

Annual review of biochemistry·2026
Same journal

Nucleocytoplasmic Transport.

Annual review of biochemistry·2026
Same journal

Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.

Annual review of biochemistry·2026
Same journal

Shaping of the Infant Gut Microbiome by Milk Oligosaccharides.

Annual review of biochemistry·2026
Same journal

Proteostasis Deregulation by Metabolism Drives the Hallmarks of Cancer.

Annual review of biochemistry·2026
Same journal

JoAnne Stubbe's Radical Path: A Story of Passion, Curiosity, and Persistence.

Annual review of biochemistry·2026
See all related articles

Related Experiment Video

Updated: Feb 12, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
10:08

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

12.1K

Regulated Proteolysis in Bacteria.

Samar A Mahmoud1, Peter Chien1

  • 1Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, Massachusetts 01003, USA; email: smahmoud@umass.edu , pchien@biochem.umass.edu.

Annual Review of Biochemistry
|April 13, 2018
PubMed
Summary
This summary is machine-generated.

Bacteria use AAA+ proteases for regulated proteolysis, degrading proteins selectively via adaptors. This process is crucial for cell quality control and adapting to growth changes.

Keywords:
AAA+ proteasesClpPClpXLon

More Related Videos

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

11.1K
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
14:23

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Published on: August 27, 2012

20.0K

Related Experiment Videos

Last Updated: Feb 12, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
10:08

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

12.1K
Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

11.1K
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
14:23

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Published on: August 27, 2012

20.0K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Microbiology

Background:

  • Regulated proteolysis is essential for all life, with bacteria employing energy-dependent AAA+ proteases.
  • These proteases degrade misfolded and regulatory proteins, requiring high specificity and selectivity.
  • Protein degradation is linked to cellular quality control, cell cycle, and physiological transitions.

Purpose of the Study:

  • To review recent advancements in understanding bacterial regulated proteolysis.
  • To discuss the function of AAA+ proteases during balanced and changing growth conditions.
  • To explore mechanisms controlling protease selectivity and substrate recognition.

Main Methods:

  • Literature review of recent research on bacterial proteolysis.
  • Analysis of AAA+ protease mechanisms and substrate specificity.
  • Examination of adaptor proteins and their role in selectivity.

Main Results:

  • AAA+ proteases play critical roles in both stable growth and adaptive responses in bacteria.
  • Protease selectivity is achieved through complex mechanisms, including adaptor proteins.
  • Coupling recognition determinants with modifying agents is a common strategy for regulated degradation.

Conclusions:

  • Regulated proteolysis in bacteria is a sophisticated process vital for cellular function and adaptation.
  • AAA+ proteases, modulated by adaptors, ensure precise protein degradation.
  • Understanding these mechanisms offers insights into bacterial physiology and potential therapeutic targets.