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Related Concept Videos

Adhesion01:14

Adhesion

44.6K
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
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Metallic Solids02:37

Metallic Solids

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Metallic solids such as crystals of copper, aluminum, and iron are formed by metal atoms. The structure of metallic crystals is often described as a uniform distribution of atomic nuclei within a “sea” of delocalized electrons. The atoms within such a metallic solid are held together by a unique force known as metallic bonding that gives rise to many useful and varied bulk properties.
All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and malleability....
20.9K
Structures of Solids02:22

Structures of Solids

18.2K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
18.2K
Molecular and Ionic Solids02:54

Molecular and Ionic Solids

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Crystalline solids are divided into four types: molecular, ionic, metallic, and covalent network based on the type of constituent units and their interparticle interactions.
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...
20.2K
Network Covalent Solids02:18

Network Covalent Solids

16.2K
Network covalent solids contain a three-dimensional network of covalently bonded atoms as found in the crystal structures of nonmetals like diamond, graphite, silicon, and some covalent compounds, such as silicon dioxide (sand) and silicon carbide (carborundum, the abrasive on sandpaper). Many minerals have networks of covalent bonds.
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
16.2K
Molecular Comparison of Gases, Liquids, and Solids02:26

Molecular Comparison of Gases, Liquids, and Solids

55.6K
Particles in a solid are tightly packed together (fixed shape) and often arranged in a regular pattern; in a liquid, they are close together with no regular arrangement (no fixed shape); in a gas, they are far apart with no regular arrangement (no fixed shape). Particles in a solid vibrate about fixed positions (cannot flow) and do not generally move in relation to one another; in a liquid, they move past each other (can flow) but remain in essentially constant contact; in a gas, they move...
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Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
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Albumin biofunctionalization to minimize the Staphylococcus aureus adhesion on solid substrates.

María Laura Martín1, Valeria Pfaffen1, Laura E Valenti1

  • 1Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Fisicoquímica, Ciudad Universitaria, X5000HUA, Córdoba, Argentina; CONICET, Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), Ciudad Universitaria, X5000HUA, Córdoba, Argentina.

Colloids and Surfaces. B, Biointerfaces
|April 13, 2018
PubMed
Summary

This study optimized surface biofunctionalization to prevent Staphylococcus aureus (S. aureus) adhesion on medical devices. Thermally treated albumin surfaces significantly minimized S. aureus colonization, reducing nosocomial infection risks.

Keywords:
Adsorption-desorption processBacterial adhesionFactorial design of experimentsPartially denatured albuminProtein coated substrateSurface protein relaxation

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Area of Science:

  • Biomaterials Science
  • Microbiology
  • Surface Chemistry

Background:

  • Staphylococcus aureus is a leading cause of nosocomial infections, primarily through biofilm formation on biomedical devices.
  • Bacterial adhesion is the critical initial step in biofilm pathogenesis, making its inhibition a key preventative strategy.
  • Developing effective surface biofunctionalization methods is crucial for reducing device-associated infections.

Purpose of the Study:

  • To optimize a surface biofunctionalization strategy for inhibiting Staphylococcus aureus adhesion on solid substrates.
  • To investigate the relationship between albumin adsorption-desorption dynamics and bacterial adhesion.
  • To design a novel biofunctionalization approach using modified albumin to prevent S. aureus colonization.

Main Methods:

  • Utilized a factorial design of experiments to study albumin adsorption-desorption kinetics under varying conditions (protein concentration, pH, flow rate, time).
  • Evaluated albumin adsorption, desorption, and initial adsorption rates on hydrophilic and hydrophobic substrates.
  • Correlated albumin surface properties with the adhesion of live S. aureus to biofunctionalized substrates.

Main Results:

  • Identified a correlation between albumin surface relaxation and S. aureus adhesion.
  • Demonstrated that surface perturbation of native albumin structure inhibits bacterial adhesion.
  • Developed a biofunctionalization strategy using thermally treated albumin (rich in β-sheet and unordered structures) via dipping.

Conclusions:

  • Surface biofunctionalization with thermally treated albumin effectively minimizes Staphylococcus aureus adhesion.
  • Altering albumin's secondary structure on solid substrates is key to preventing bacterial colonization.
  • This strategy offers a promising approach to reduce S. aureus-related nosocomial infections on biomedical devices.