Related Experiment Videos
Biochemical effects of gossypol in isolated mitochondria: monovalent cations and ATPase activity
F Martínez1, S Gamboa, V Díaz-Sánchez
1Departamento de Bioquímica, Facultad de Medicina, México, D.F. México.
The International Journal of Biochemistry
|January 1, 1988
Abstract:
1. The effect of gossypol acetic acid upon the energy-producing system of isolated liver mitochondria was studied. Gossypol was found to exert an uncoupling effect. 2. Oxygen consumption and ATPase activity were stimulated only when a monovalent alkali metal cations was present. 3. The mitochondrial proton gradient, produced by the enzymatic hydrolysis of ATP, was lower in the gossypol-treated mitochondria than in the control experiments. 4. It is proposed that gossypol stimulates oxygen consumption and the ATPase activity in mitochondria by a protonophoretic effect and a cation translocation process.