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Genome Sequences of Ilzat and Eleri, Two Phages Isolated Using Microbacterium foliorum NRRL B-24224

Ilzat Ali1,2, Acacia Eleri Jones3, Aleem Mohamed2

  • 1Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, Maryland, USA.

Genome Announcements
|April 14, 2018
PubMed

Insights

Newly discovered bacteriophages, Ilzat and Eleri, infect Microbacterium foliorum. These Siphoviridae phages exhibit similar genome characteristics and share significant nucleotide sequence identity, indicating a close relationship.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria and are increasingly explored for therapeutic applications.
  • The Siphoviridae family is characterized by long, non-contractile tails.
  • Microbacterium foliorum is a bacterial species that can be a target for phage therapy.

Purpose of the Study:

  • To isolate and characterize novel bacteriophages infecting Microbacterium foliorum.
  • To analyze the genomic features and sequence identity of newly isolated phages Ilzat and Eleri.

Main Methods:

  • Isolation and purification of bacteriophages from environmental samples.
  • Genome sequencing and comparative analysis of bacteriophage DNA.
  • Bioinformatic analysis of genome length, G+C content, and overall architecture.

Main Results:

  • Two novel bacteriophages, Ilzat and Eleri, belonging to the Siphoviridae family were successfully isolated.
  • Both phages infect the bacterial strain Microbacterium foliorum NRRL B-24224.
  • The genomes of Ilzat and Eleri are similar in length, G+C content, and architecture, sharing 62.9% nucleotide sequence identity.

Conclusions:

  • Bacteriophages Ilzat and Eleri represent new additions to the Siphoviridae family.
  • The high sequence identity suggests a common ancestry or recent divergence between Ilzat and Eleri.
  • These findings contribute to the understanding of phage diversity and host specificity within the Microbacterium genus.

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