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Updated: Feb 12, 2026

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
Published on: March 20, 2013
Lymphatic exosomes promote dendritic cell migration along guidance cues
Markus Brown1,2, Louise A Johnson3, Dario A Leone1
1Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
Abstract:
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendritic cells. In this study, we report that LECs also release basolateral exosome-rich endothelial vesicles (EEVs) that are secreted in greater numbers in the presence of inflammatory cytokines and accumulate in the perivascular stroma of small lymphatic vessels in human chronic inflammatory diseases. Proteomic analyses of EEV fractions identified >1,700 cargo proteins and revealed a dominant motility-promoting protein signature. In vitro and ex vivo EEV fractions augmented cellular protrusion formation in a CX3CL1/fractalkine-dependent fashion and enhanced the directional migratory response of human dendritic cells along guidance cues. We conclude that perilymphatic LEC exosomes enhance exploratory behavior and thus promote directional migration of CX3CR1-expressing cells in complex tissue environments.
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