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Updated: Feb 12, 2026
08:12
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
Published on: March 20, 2013
12.2K
Lymphatic exosomes promote dendritic cell migration along guidance cues
Markus Brown1,2, Louise A Johnson3, Dario A Leone1
1Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
The Journal of Cell Biology
|April 14, 2018
Summary
Lymphatic endothelial cells release vesicles that promote cell movement. These findings reveal a new mechanism for how immune cells navigate tissues during inflammation.
Area of Science:
- Immunology
- Cell Biology
- Vascular Biology
Background:
- Lymphatic endothelial cells (LECs) are known to guide immune cell migration via chemokines.
- The role of other secreted factors from LECs in immune cell trafficking is less understood.
Purpose of the Study:
- To investigate the role of exosome-rich endothelial vesicles (EEVs) released by LECs in dendritic cell migration.
- To characterize the cargo and function of LEC-derived EEVs in inflammatory conditions.
Main Methods:
- Proteomic analysis of EEV fractions isolated from LECs.
- In vitro and ex vivo assays to assess the effect of EEVs on dendritic cell migration and protrusion formation.
- Analysis of EEVs in human chronic inflammatory disease tissues.
Main Results:
- LECs secrete EEVs, particularly under inflammatory conditions.
- Proteomic analysis revealed over 1,700 cargo proteins in EEVs, with a significant motility-promoting signature.
- EEVs enhanced dendritic cell protrusion formation and directional migration in a CX3CL1/fractalkine-dependent manner.
- EEVs were found to accumulate in the perivascular stroma of lymphatic vessels in human chronic inflammatory diseases.
Conclusions:
- Perilymphatic LEC exosomes (EEVs) are secreted in response to inflammation.
- EEVs contain proteins that promote cell motility.
- LEC-derived EEVs enhance dendritic cell exploratory behavior and directional migration in complex tissue environments, particularly for CX3CR1-expressing cells.
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