Related Experiment Video
Updated: Feb 12, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
Published on: August 27, 2014
Light-Induced Remodeling of Physically Crosslinked Hydrogels Using Near-IR Wavelengths
Congcong Zhu1, Christopher J Bettinger1,2,3
1Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Abstract:
Poly([6-bromo-7-hydroxycoumarin-4-yl]methyl methacrylate)-based triblock copolymers were synthesized by first preparing PMAA-PEG-PMAA triblocks using ATRP. 6-Bromo-4-chloromethyl-7-hydroxycoumarin was conjugated to PMAA-PEG-PMAA using 1,8-diazabicycloundec-7-ene (DBU). Rheological measurements were conducted using a HR-2 rheometer with a UV illumination accessory (TA Instruments). Single-photon uncaging was performed as previously described.20 Two-photon uncaging was performed using a LSM (Zeiss) equipped with a Ti: Sapphire laser (Coherent). All values reported as mean ± std. dev. unless otherwise stated. See Supporting Information for experimental details.
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