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Updated: Feb 12, 2026

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
Rieske non-heme iron-dependent oxygenases catalyse diverse reactions in natural product biosynthesis
Christopher Perry1, Emmanuel L C de Los Santos, Lona M Alkhalaf
1Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK. g.l.challis@warwick.ac.uk l.alkhalaf.1@warwick.ac.uk.
Abstract:
Covering: up to the end of 2017 The roles played by Rieske non-heme iron-dependent oxygenases in natural product biosynthesis are reviewed, with particular focus on experimentally characterised examples. Enzymes belonging to this class are known to catalyse a range of transformations, including oxidative carbocyclisation, N-oxygenation, C-hydroxylation and C-C desaturation. Examples of such enzymes that have yet to be experimentally investigated are also briefly described and their likely functions are discussed.
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