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Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
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Bevacizumab and near infrared probe conjugated iron oxide nanoparticles for vascular endothelial growth factor

Run Lin1, Jing Huang, Liya Wang

  • 1Department of Radiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.

Biomaterials Science
|April 14, 2018
PubMed

Insights

A novel dual-modality imaging probe targets vascular endothelial growth factor (VEGF) in cancers. This probe, comprising iron oxide nanoparticles and an anti-VEGF antibody, shows enhanced tumor accumulation and improved MRI contrast for cancer detection and treatment monitoring.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Imaging

Background:

  • Vascular endothelial growth factor (VEGF) is crucial in cancer development and progression.
  • Targeting VEGF offers a strategy for cancer detection and anti-angiogenesis treatment monitoring.
  • Developing effective molecular imaging probes is essential for precision oncology.

Purpose of the Study:

  • To develop a multimodal imaging probe targeting VEGF for cancer detection.
  • To evaluate the efficacy of the probe in imaging and monitoring anti-angiogenesis therapy.
  • To assess the dual-modality capabilities (optical and MR imaging) of the developed probe.

Main Methods:

  • Conjugation of a near-infrared dye (NIR830)-labeled anti-VEGF antibody (Bevacizumab) to iron oxide nanoparticles (IONPs).
  • Investigation of targeted cell uptake using VEGF-overexpressing 4T1 breast cancer cells and blocking assays.
  • In vivo studies in mice bearing 4T1 tumors to assess tumor accumulation via MRI and optical imaging.

Main Results:

  • The NIR830-Bevacizumab-IONPs demonstrated receptor-mediated cell uptake in VEGF-overexpressing cells.
  • Systemic administration led to higher accumulation of targeted IONPs in tumors compared to non-targeted IONPs.
  • T2-weighted MRI showed a significant signal intensity change in tumors treated with targeted IONPs (52.4%) versus controls (26.9%).

Conclusions:

  • NIR830-Bevacizumab-IONPs serve as a feasible and effective VEGF-targeting dual-modality molecular imaging probe.
  • The probe shows potential for imaging VEGF-overexpressing cancers.
  • This technology could enable imaging-guided anti-cancer treatment delivery.

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