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A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
Published on: October 29, 2018
A nation-wide genetic survey revealed a complex population structure of Bemisia tabaci in Pakistan
Waqar Islam1, Wenzhong Lin2, Muhammad Qasim2
1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou 350002, China; Govt. of Punjab, Agriculture Department, Lahore, Pakistan; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Abstract:
The whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex distributed worldwide. In Pakistan, B. tabaci poses a serious threat to agriculture production. To understand its diversity in Pakistan, a large-scale sampling was conducted from various locations of all four provinces of the country and Mitochondrial cytochrome oxidase I (mtCOI) gene sequencing was used to determine the whiteflies genetically. The study revealed the presence of five different cryptic species in Pakistan namely Asia II-1, Asia II-5, Asia II-7, Asia II-8 and MEAM-1, respectively. Among them, Asia II-1, which was previously reported from a few areas in the country, had been found now to be prevalent all over the country covering 88.7% of all the sequenced samples. Based on the mtCOI sequences and genetic distance analyses, the diversity of Asia II-1 was much greater than all other cryptic species, which exist only in small patches.
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