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Updated: Feb 11, 2026

Preparing Silica Aerogel Monoliths via a Rapid Supercritical Extraction Method
Published on: February 28, 2014
Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography
Dana Moravcová1, Josef Planeta1, Alistair W T King2
1Czech Academy of Sciences, Institute of Analytical Chemistry, Veveri 97, Brno, 60200, Czech Republic.
Abstract:
A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000-174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.
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