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DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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The DNA Replication Fork01:02

The DNA Replication Fork

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S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
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Chromosome Replication02:31

Chromosome Replication

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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Replication in Prokaryotes02:35

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Related Experiment Video

Updated: Feb 11, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

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DNA replication licensing in stem cells: Gatekeeping the commitment to proliferation.

Hilary A Coller1

  • 1Molecular, Cell and Developmental Biology, Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA hcoller@ucla.edu.

The Journal of Cell Biology
|April 15, 2018
PubMed
Summary
This summary is machine-generated.

DNA replication licensing is crucial for cell proliferation. A new method shows intestinal stem cells with cancer mutations are unlicensed, suggesting licensing limits proliferation commitment.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • DNA replication licensing is a critical checkpoint for cell division.
  • Understanding licensing in stem cells is key to understanding tissue homeostasis and cancer development.

Purpose of the Study:

  • To develop and apply a method for assessing DNA replication licensing in intact tissues.
  • To investigate the role of DNA replication licensing in intestinal stem cells and their response to cancer-causing mutations.

Main Methods:

  • Development of a novel method to quantify DNA replication licensing status in tissue samples.
  • Application of the method to analyze intestinal crypts from wild-type and genetically modified mouse models.

Main Results:

  • Intestinal stem cells in wild-type crypts are largely unlicensed for DNA replication.
  • Stem cells within crypts harboring cancer-causing mutations exhibit a significantly different licensing profile.
  • DNA replication licensing appears to be a rate-limiting factor in the commitment to proliferation in intestinal stem cells.

Conclusions:

  • The developed method provides a valuable tool for studying DNA replication licensing in complex tissues.
  • DNA replication licensing status is dynamically regulated in intestinal stem cells.
  • Impaired DNA replication licensing may contribute to uncontrolled proliferation in cancer.