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Related Experiment Videos

A functional test for protein S activity in plasma.

P van de Waart1, K T Preissner, J R Bechtold

  • 1Clinical Research Unit for Blood Coagulation and Thrombosis, Max-Planck-Gesellschaft, Justus-Liebig-Universität, Giessen, Federal Republic of Germany.

Thrombosis Research
|November 15, 1987
PubMed
Summary
This summary is machine-generated.

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A new clotting assay measures Protein S (PrS) functional activity, aiding in the diagnosis of PrS-deficiency and related thromboembolism. This sensitive test is suitable for routine clinical use.

Area of Science:

  • Hematology
  • Clinical Chemistry
  • Biochemistry

Background:

  • Protein S (PrS) is a crucial cofactor for activated Protein C (APC) in regulating coagulation.
  • PrS-deficiency is linked to an increased risk of thromboembolism.
  • Accurate assessment of PrS functional activity is vital for clinical diagnosis.

Purpose of the Study:

  • To develop and validate a novel one-stage clotting assay for measuring Protein S functional activity in plasma.
  • To assess the sensitivity, specificity, and reproducibility of the developed assay.
  • To evaluate the utility of the assay in identifying patients with PrS-deficiency and those on anticoagulant therapy.

Main Methods:

  • A factor Xa-initiated clotting assay was designed using adsorbed substrate plasma, patient plasma, purified prothrombin, APC, phospholipids, and CaCl2.

Related Experiment Videos

  • Assay specificity was confirmed using anti-PrS antibodies and purified PrS reconstitution.
  • Inter-assay and intra-assay variations were determined (n=20).

    • Main Results:

    • The assay demonstrated PrS-specific prolongation of clotting times in the presence of APC.
    • The functional assay exhibited acceptable reproducibility with variations of 11.7% (inter-assay) and 10.1% (intra-assay).
    • In unselected patients (n=34), PrS activity was 95.8 ± 16.5%, while patients on oral anticoagulants (n=32) showed 21.1 ± 10.8% residual activity.

    Conclusions:

    • The developed one-stage clotting assay is a sensitive and reliable method for assessing Protein S functional activity.
    • This assay can be routinely performed and is effective in detecting PrS-deficiency.
    • The assay provides valuable diagnostic information for patients with coagulation disorders and those undergoing anticoagulant therapy.