Related Experiment Video
Updated: Feb 11, 2026

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
Published on: October 31, 2012
Guided bone regeneration in calvarial critical size bony defect using a double-layer resorbable collagen membrane
Roy Abou Fadel1, Rawad Samarani2, Carole Chakar2
1Department of Periodontology, Faculty of Dentistry, Université Saint Joseph, Beirut, Lebanon. roy.aboufadel@usj.edu.lb.
Purpose:
The aim of the present study was to evaluate histologically and histomorphometrically the bone regeneration in critical size calvarial defects in rats grafted with either a deproteinized bovine bone mineral (DBBM) alone or in combination with a single or double layer of native bilayer collagen membrane (NBCM). The secondary objective was to evaluate histologically and histomorphometrically the residual DBBM in these defects.
Material And Methods:
Thirty-two Wistar rats were divided into two groups: a control group of 16 rats with two critical size calvarial defects (CSD) of 5 mm performed each on either side of the median sagittal suture, where the frontal defect remained without any filling (negative control), while the occipital defect (positive control) was filled with DBBM; and then a test group of 16 rats, with two CSD filled with DBBM and covered by either a single (SM) or a double layer (DM) of NBCM. The animals were sacrificed at 4 and 8 weeks.
Results:
At 1 month, the histological and histomorphometric analysis showed new bone formation (NBF) in the defects that received only DBBM, DBBM+DM, and DBBM+SM (11.5, 17.3, and 22.7%, respectively), while the negative control defects showed only 0.4% of new bone formation. At 2 months, the histological and histomorphometric analysis showed NBF in the defects that received only DBBM, DBBM+DM, and DBBM+SM (16.8, 24.5, and 37%, respectively), while the negative control defects showed only 0.9% of new bone formation. The residual xenogeneic material (RXM) was higher in defects covered by SM (30.2% at 1 month and 25.3% at 2 months) or DM (32.5% at 1 month and 28.5% at 2 months) compared with defects that were not covered by membranes (15.3% at 1 month and 9.4% at 2 months).
Conclusions:
This study demonstrated that GBR with a xenogeneic material in rat calvarial (CSD) of 5 mm requires the application of resorbable collagen membranes in either single or double layer, and a single layer alone is sufficient to promote this regeneration.
Related Concept Videos
Critical Region, Critical Values and Significance Level
In hypothesis testing, a sample statistic is converted to a test statistic using z, t, or chi-square distribution. A critical region is an area under the curve in probability distributions demarcated by the critical value. When the test statistic falls in this region, it suggests that the null hypothesis must be rejected. As this region contains all those values of the...
Critical Values
Lumber Defects
Shakes are minor fractures that run along or across the wood's annual rings, while wane is...
Fibril-associated Collagen
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Critical Thinking
Colleges and universities are...
Critical Thinking I

