NMR Spectroscopy Protocols for Food Metabolomics Applications
Evangelia Ralli1, Maria Amargianitaki1, Efi Manolopoulou1
1NMR Laboratory, Chemistry Department, University of Crete, Heraklion, Crete, Greece.
Abstract:
NMR spectroscopy has become an indispensable tool for the metabolic profiling of foods and food products. In the present protocol, we report an analytical approach based on liquid-state NMR for the determination of polar and nonpolar metabolites in some common liquid (wine, spirits, juice) and solid (cheese, coffee, honey) foods. Although the diversity of foods precludes the use of a single protocol, with small modifications, the proposed methodologies can be adapted to a broader range of foodstuffs.
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