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Updated: Feb 11, 2026
08:31
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
Published on: April 10, 2020
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Acyl donors for native chemical ligation.
Bingjia Yan1, Weiwei Shi1, Linzhi Ye1
1Tsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Beijing Advanced Innovation Center for Structural Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Current Opinion in Chemical Biology
|April 15, 2018
Summary
Native chemical ligation (NCL) is key for protein synthesis. Recent efforts focus on improving C-terminal acyl donors, expanding NCL
Area of Science:
- Chemical Biology
- Synthetic Chemistry
- Biochemistry
Background:
- Native chemical ligation (NCL) is a critical method for synthesizing proteins.
- Expanding the scope of NCL is essential for advancing protein chemical synthesis.
Purpose of the Study:
- To review recent advancements in designing C-terminal acyl donors for NCL.
- To highlight progress in precursors and surrogates for NCL reactions.
Main Methods:
- Review of recent literature on NCL.
- Analysis of novel C-terminal acyl donor designs.
- Evaluation of precursor and surrogate strategies.
Main Results:
- Significant progress has been made in tuning C-terminal acyl donors.
- New precursors and surrogates have been developed to enhance NCL efficiency.
- These advances broaden the applicability of protein chemical synthesis.
Conclusions:
- Optimized C-terminal acyl donors are crucial for the future of NCL.
- Continued innovation in this area will drive progress in synthesizing complex proteins.
- This review provides insights into emerging trends in protein synthesis.
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