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Related Concept Videos

Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

19.6K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
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Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

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Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Elongation Factors02:35

Transcription Elongation Factors

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Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
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Transcription Elongation Factors02:35

Transcription Elongation Factors

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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Related Experiment Video

Updated: Feb 11, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

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Targeting Intrinsically Disordered Transcription Factors: Changing the Paradigm.

K Tsafou1, P B Tiwari1, J D Forman-Kay2

  • 1Department of Oncology and Pediatrics, Georgetown University, 3970 Reservoir Road Northwest, Washington, DC 20057, USA.

Journal of Molecular Biology
|April 16, 2018
PubMed
Summary
This summary is machine-generated.

Intrinsically disordered transcription factors (TFs) are crucial for cell function but hard to study. New methods now allow detailed characterization and drug development for these dynamic proteins.

Keywords:
EWS-FLI1c-Mycdrug developmentintrinsically disordered proteinsp53transcription factors

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

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Related Experiment Videos

Last Updated: Feb 11, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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Area of Science:

  • Biochemistry and Molecular Biology
  • Structural Biology
  • Genetics

Background:

  • Intrinsically disordered proteins (IDPs) challenge traditional structure-function paradigms.
  • IDPs lack fixed structures but exhibit dynamic functions, kinetics, and affinities.
  • Intrinsically disordered transcription factors (TFs) are poorly understood due to mixed sequences.

Purpose of the Study:

  • To introduce the biophysical properties of intrinsically disordered TFs.
  • To discuss computational and experimental efforts in understanding disordered TFs.
  • To explore TF targeting strategies and drug development.

Main Methods:

  • Literature review of biophysical properties of intrinsically disordered TFs.
  • Discussion of computational and experimental approaches.
  • Analysis of successful TF targeting strategies.

Main Results:

  • IDPs can be functional without a fixed 3D structure.
  • TF modulation by small molecules was previously challenging.
  • Evolving technologies enable detailed TF structure-function analysis.
  • Successful strategies have overcome the 'undruggability' of TFs.

Conclusions:

  • Intrinsically disordered TFs play significant roles in biology and disease.
  • Advances in characterization offer new drug development avenues.
  • Further research is needed to fully understand disordered TF structure-function relationships.