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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
Published on: December 28, 2015
The bcl-2 gene is rearranged in many diffuse B-cell lymphomas
A C Aisenberg1, B M Wilkes, J O Jacobson
1Department of Medicine, Massachusetts General Hospital, Boston 02114.
Blood
|April 1, 1988
Summary
Rearrangement of the bcl-2 gene was detected in various B cell lymphomas, particularly those of follicle center cell lineage. This finding highlights the bcl-2 gene
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The bcl-2 gene, located on chromosome 18, is frequently rearranged in certain lymphomas.
- Previous studies linked bcl-2 gene rearrangement to the 14;18 chromosome translocation in follicular lymphomas.
Purpose of the Study:
- To investigate the incidence of bcl-2 gene rearrangement in non-Hodgkin's lymphoma (NHL) subtypes.
- To determine the association between bcl-2 gene rearrangement and the 14;18 translocation in NHL.
Main Methods:
- Southern blotting was employed to detect bcl-2 gene rearrangements.
- The study analyzed 104 NHL cases, 24 B cell chronic lymphocytic leukemia (B-CLL) cases, and 14 T cell malignancy cases.
- Hybridization with immunoglobulin heavy chain joining region probes confirmed the 14;18 translocation.
Main Results:
- bcl-2 gene rearrangement was observed in 30% of follicular lymphomas and 19% of diffuse lymphomas of follicle center cell lineage.
- The 14;18 translocation was confirmed in 18 of 19 rearranged samples.
- The bcl-2 gene remained in germline configuration in lymphomas not derived from follicle center cells, including B-CLL and T cell neoplasms.
Conclusions:
- bcl-2 gene rearrangement is a common event in B cell lymphomas, including those with diffuse morphology.
- The 14;18 translocation is the primary mechanism for bcl-2 rearrangement in these lymphomas.
- The findings emphasize the significance of bcl-2 gene alterations in the pathogenesis of various B cell lymphomas.
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