A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase
Yue Wang1, Fengge Zhai1, Yasushi Hasebe2
1School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China.
Bioelectrochemistry (Amsterdam, Netherlands)
|April 16, 2018
Summary
A new phenol biosensor was developed using tyrosinase immobilized on graphene oxide. This highly sensitive and stable biosensor offers a wide linear range for detecting catechol, crucial for environmental and biological monitoring.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Materials Science
Background:
- Phenol and its derivatives are common environmental pollutants.
- Developing sensitive and selective detection methods for phenols is crucial.
- Tyrosinase is an enzyme with high specificity for phenolic compounds.
Purpose of the Study:
- To fabricate and characterize a novel biosensor for phenol detection.
- To investigate the covalent immobilization of tyrosinase onto graphene oxide.
- To evaluate the analytical performance of the developed biosensor.
Main Methods:
- Covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) using glutaraldehyde (GA).
- Surface morphology analysis using atomic force microscopy (AFM) and field-emission scanning electron microscopy (FE-SEM).
- Electrochemical characterization and performance evaluation of the biosensor.
Main Results:
- The TYR/GA/GO/GCE biosensor demonstrated excellent stability, reproducibility, and sensitivity.
- A wide linear sensing range for catechol was observed from 5×10⁻⁸M to 5×10⁻⁵M.
- A low detection limit of 3×10⁻⁸M and a maximum current (Imax) of 65.8μA were achieved.
Conclusions:
- The developed tyrosinase-based biosensor offers a promising platform for sensitive and reliable phenol detection.
- The use of graphene oxide enhances the biosensor's performance characteristics.
- This biosensor has potential applications in environmental monitoring and biochemical analysis.
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