Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

17.8K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.8K
Health Information Technology and Healthcare Information System01:30

Health Information Technology and Healthcare Information System

3.4K
Health Information Technology (HIT)
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:
3.4K
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

3.3K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
3.3K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

15.0K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
15.0K
Applications of Logarithms01:28

Applications of Logarithms

288
Logarithmic functions are powerful tools for simplifying the mathematical representation of phenomena involving exponential changes. Their ability to convert multiplicative relationships into additive ones is especially valuable in various scientific and engineering contexts. One notable application of logarithms is measuring sound intensity, specifically through the decibel (dB) scale used in acoustics.Sound intensity levels vary over an extensive range, from the faintest audible whisper to...
288
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zein-Ceria Hybrid Microparticles Enable Long-Term ROS-Scavenging Oxygenation for Osteogenic Microtissues Engineering.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Sex-related differences in femorotibial cartilage thickness: A matched pair analysis.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2026
Same author

A Synovium-on-Chip Platform to Study Multicellular Interactions in Arthritis.

Advanced healthcare materials·2026
Same author

Tailorable Biofunctionalization of Poly(acrylamide) Hydrogels via Firefly Luciferin-Bioinspired Click Ligation Accelerates Cell Attachment, Spreading, and Proliferation.

ACS applied materials & interfaces·2026
Same author

Tailoring human joint-on-a-chip: from biological principles, materials, to disease modeling.

Materials today. Bio·2026
Same author

Arresting Microphase Separation Encodes Material Mechanics by Sculpting Microarchitectures and Local Polymer Enrichment.

Advanced materials (Deerfield Beach, Fla.)·2025
Same journal

Bacterial spores as a modular platform for the production of amyloids for materials.

Trends in biotechnology·2026
Same journal

The oriGen case and Mexico's regulatory blind spots in genomic biobanking.

Trends in biotechnology·2026
Same journal

A caspase-3-activated protein expression system for apoptosis visualization and apoptosis-pyroptosis conversion to boost antitumor activity.

Trends in biotechnology·2026
Same journal

Over 4 months of ethylene production using solid-state photosynthetic cell factories.

Trends in biotechnology·2026
Same journal

Closing the nitrogen loop in groundwater with biohybrid technologies.

Trends in biotechnology·2026
Same journal

Engineering environmental bacteria for whole-cell PET hydrolysis and assimilation.

Trends in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Feb 11, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

4.0K

Single-Cell Microgels: Technology, Challenges, and Applications.

Tom Kamperman1, Marcel Karperien2, Séverine Le Gac3

  • 1Department of Developmental BioEngineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Drienerlolaan 5, 7522NB Enschede, The Netherlands. Electronic address: https://twitter.com/DBE_MIRA.

Trends in Biotechnology
|April 17, 2018
PubMed
Summary
This summary is machine-generated.

Single-cell microgels mimic natural cell environments, offering advantages for research and medicine. This technology facilitates cell manipulation, culture, and analysis in 3D, advancing life sciences.

Keywords:
3D culturehydrogelsmicroenvironmentsmicrofabricationmicrofluidicsregenerative medicinesingle cell analysis

More Related Videos

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting
04:58

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting

Published on: May 3, 2024

1.8K
The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System
11:27

The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System

Published on: April 25, 2012

15.9K

Related Experiment Videos

Last Updated: Feb 11, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

4.0K
Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting
04:58

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting

Published on: May 3, 2024

1.8K
The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System
11:27

The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System

Published on: April 25, 2012

15.9K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Single-cell-laden microgels serve as engineered microniches, mimicking the cell's natural pericellular matrix.
  • Recent advancements allow single-cell encapsulation in sub-100-μm microgels, creating physiologically relevant environments.
  • These microgels offer minimal mass transport limitations and favorable pharmacokinetics.

Purpose of the Study:

  • To review the latest trends, technical challenges, and breakthroughs in single-cell microgel technology.
  • To discuss the diverse applications of single-cell microgels in life sciences.
  • To present a future outlook for single-cell microgel technology.

Main Methods:

  • Encapsulation of single cells within microgel structures.
  • Characterization of microgel properties and cell behavior within the microenvironment.
  • Review of existing literature and technological advancements.

Main Results:

  • Single-cell microgels provide facile manipulation, culture, and analysis of individual cells in 3D.
  • These microgels offer physiologically relevant microniches with improved mass transport and pharmacokinetic properties.
  • The technology has broad applicability in pharmacological screenings, regenerative medicine, and biological research.

Conclusions:

  • Single-cell microgel technology is a powerful tool for creating controlled cellular microenvironments.
  • It holds significant promise for advancing various fields within the life sciences.
  • Continued innovation is expected to expand the capabilities and applications of this technology.