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Related Concept Videos

Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Histone Modification02:32

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Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
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Updated: Feb 11, 2026

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
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Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

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Injectable polypeptide hydrogels via methionine modification for neural stem cell delivery.

A L Wollenberg1, T M O'Shea2, J H Kim2

  • 1Departments of Bioengineering, Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095-1600, USA.

Biomaterials
|April 17, 2018
PubMed
Summary
This summary is machine-generated.

Injectable methionine-based hydrogels support neural stem cell survival and function. These novel biomaterials show promise for central nervous system repair strategies.

Keywords:
BiomaterialsBrainCell transplantationHydrogelsNeural stem cellsPolypeptides

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Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Regenerative Medicine

Background:

  • Injectable hydrogels are promising for neural stem/progenitor cell (NSPC) transplantation in central nervous system (CNS) disorders.
  • Developing biomaterials that support NSPC survival, stemness, and integration is crucial for CNS repair.

Purpose of the Study:

  • To develop and characterize injectable diblock copolypeptide hydrogels (DCH) based on modified l-methionine (Met) for NSPC transplantation.
  • To evaluate the in vitro and in vivo behavior of NSPCs within these Met-based DCH.

Main Methods:

  • Fabrication of Met-based DCH via post-polymerization modification and incorporation of amino acid comonomers.
  • Assessment of hydrogel self-healing properties, mechanical characteristics, and stability in various media.
  • Evaluation of murine NSPC survival, stemness, and multipotency in vitro within DCH.
  • In vivo transplantation of NSPCs in DCH into uninjured murine forebrain and assessment of cell fate and host tissue integration.

Main Results:

  • Met-based DCH exhibited self-healing properties with tunable mechanical characteristics.
  • Non-ionic Met-sulfoxide formulations (DCHMO) demonstrated stable mechanical properties and supported NSPC survival comparable to standard culture.
  • DCHMO provided cell non-fouling properties and superior preservation of NSPC stemness and multipotency in vitro.
  • In vivo, transplanted NSPCs in DCHMO remained localized, integrated with host tissue, and supported axonal growth.

Conclusions:

  • Met-based DCH are suitable injectable biomaterials for NSPC transplantation.
  • These hydrogels support NSPC survival, stemness, and promote neural integration in vivo.
  • Met-based DCH represent a promising platform for advancing NSPC-based therapies for CNS injury and disease.