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lncRNA - Long Non-coding RNAs02:39

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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Roles of Non-Coding RNA in Sugarcane-Microbe Interaction.

Flávia Thiebaut1, Cristian A Rojas2, Clícia Grativol3

  • 1Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil. flaviabqi@gmail.com.

Non-Coding RNA
|April 17, 2018
PubMed
Summary
This summary is machine-generated.

Sugarcane microRNAs (miRNAs) play a key role in plant-microbe interactions. This study reveals miR408

Keywords:
Acidovorax avenaediazotrophic bacteriamicroRNApathogensiRNA

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Area of Science:

  • Plant molecular biology
  • Genomics
  • Biochemistry

Background:

  • Non-coding RNAs, including microRNAs (miRNAs), are crucial regulators in plant-microbe interactions.
  • The specific roles of sugarcane miRNAs in disease response remain largely unexplored.

Purpose of the Study:

  • To investigate the function of sugarcane miRNAs in response to pathogenic bacteria, specifically *Acidovorax avenae*.
  • To identify novel miRNAs and understand their regulatory mechanisms in sugarcane immunity.

Main Methods:

  • Small RNA (sRNA) transcriptome sequencing of sugarcane infected with *A. avenae*.
  • Bioinformatic analysis of miRNA and small interfering RNA (siRNA) sequences.
  • Quantitative reverse transcription-PCR (qRT-PCR) for miRNA expression validation.
  • 5' rapid amplification of cDNA ends (5'RACE) assay to confirm miRNA target cleavage.

Main Results:

  • Identified conserved and novel miRNAs and siRNAs in sugarcane infected with *A. avenae*.
  • Observed differential regulation of miR408, a copper-microRNA, which was downregulated during pathogen infection but upregulated with beneficial bacteria.
  • Confirmed miR408 targets, including a laccase, demonstrating its role in post-transcriptional gene silencing.
  • siRNAs targeting a copper-transporter gene were repressed upon *A. avenae* infection.

Conclusions:

  • Sugarcane employs specific miRNA-mediated regulatory mechanisms to distinguish between pathogenic and beneficial microorganisms.
  • miR408 is a key regulator in sugarcane's response to microbial stimuli, influencing disease resistance and beneficial interactions.
  • This study provides insights into the complex non-coding RNA-based defense strategies in plants.