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Estimation and quantification of human DNA in dental calculus: A pilot study
1Department of Oral Medicine and Radiology, Kothiwal Dental College and Research Centre, Moradabad, Uttar Pradesh, India.
Journal of Forensic Dental Sciences
|April 17, 2018
Summary
Dental calculus, a calcified plaque, contains human DNA, offering a valuable alternative for forensic identification when traditional sources like teeth or bone are unavailable. This study found DNA in 85% of samples.
Area of Science:
- Forensic Science
- Molecular Biology
- Dental Science
Background:
- DNA analysis is crucial for forensic identification, typically using teeth or bone.
- Challenges arise when remains are unavailable, poorly preserved, or inaccessible due to religious objections.
- Alternative DNA sources are needed for reliable identification.
Purpose of the Study:
- To determine if human DNA is present in dental calculus.
- To quantify the amount of human DNA within dental calculus samples.
Main Methods:
- Prospective, single-blinded pilot study involving twenty dental patients.
- Dental calculus samples collected from lingual surfaces of mandibular incisors.
- DNA extraction performed using gel electrophoresis after decontamination.
Main Results:
- Human DNA was detected in 85% of the analyzed dental calculus samples.
- The quantity of DNA recovered ranged from 21 to 37 μg/ml of dental calculus.
Conclusions:
- Dental calculus serves as a rich and viable reservoir for human DNA.
- This finding supports the potential use of dental calculus in forensic identification.
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