Effects of elevated carbon dioxide on male and female behavioural lateralization in a temperate goby

Behavioural abnormality in fishes has been proposed as a significant consequence of the increasing levels of carbon dioxide occurring in the oceans. Negative effects of elevated CO₂ have been reported for behaviours such as predator-prey interactions, foraging, hearing and behavioural lateralization. Importantly, the effects vary greatly both within and between species, and some recent studies have shown minimal effects of CO₂ on behaviour. Whether the effect of CO₂ also varies between males and females is, however, virtually unexplored. According to resource allocation theory, females are expected to be more sensitive to elevated CO₂, meaning that non-sex-specific studies may overlook ecologically important differences between the sexes. In this study, we investigated the possible differences between males and females in their response to elevated CO₂ by performing behavioural lateralization tests in adult temperate two-spotted gobies, Gobiusculus flavescens. We found that the strength of the side bias (absolute lateralization) was unaffected by the CO₂ treatment, and there was no difference between males and females. The control fish were slightly right-biased in their behavioural asymmetry (mean relative lateralization of 14). Exposure to high CO₂ affected this pattern, such that treated fish were slightly left-biased (mean relative lateralization of -10), regardless of their sex. The same results were obtained yet again when the study was repeated during a second year. We discuss our results in light of the great variation in lateralization that has been reported to depend on variables such as species, ecological settings and environmental factors.