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Updated: Feb 11, 2026

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Increasing cell-device adherence using cultured insect cells for receptor-based biosensors.

Daigo Terutsuki1, Hidefumi Mitsuno2, Takeshi Sakurai2

  • 1Department of Advanced Interdisciplinary Studies, Graduate School of Engineering, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, Japan.

Royal Society Open Science
|April 17, 2018
PubMed
Summary

Investigating insect cell-device interfaces is crucial for developing effective field-effect transistor (FET)-based biosensors. Understanding these interfaces aids in optimizing signal transfer for advanced odorant detection systems.

Keywords:
cell adhesioncell–electronic interfacecross-section polisherfield-effect transistorodorant sensorsf21 insect cell

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Area of Science:

  • Biosensor technology
  • Cell-device interface characterization
  • Insect odorant receptor applications

Background:

  • Field-effect transistor (FET)-based biosensors offer broad applications.
  • Insect (Sf21) cells expressing insect odorant receptors (ORs) show promise for odorant sensing.
  • Understanding the Sf21 cell-device interface is vital for practical bio-FET odorant sensor development.

Purpose of the Study:

  • To investigate the Sf21 cell-device interface for bio-FET odorant sensors.
  • To assess the suitability of electrode materials for cell adhesion and function.
  • To provide foundational knowledge for optimizing signal transfer in bio-FETs.

Main Methods:

  • Development of cross-sectional specimens to study cell-device interfaces.
  • Utilizing calcium imaging to verify Sf21 cell function as odorant sensor elements.
  • Analysis of cell adhesion on electrode materials.

Main Results:

  • The Sf21 cell-device interface was characterized, measuring approximately 10 nm in width.
  • Evidence suggests that Sf21 cell adhesion mechanisms may differ from other cell types.
  • Functional verification of Sf21 cells as odorant sensors on electrode materials was achieved.

Conclusions:

  • Characterizing the Sf21 cell-device interface is essential for FET-based biosensor design.
  • The findings provide insights into Sf21 cell adhesion, informing material selection.
  • This research contributes to the accurate signal detection from insect ORs using FETs.