Portable lensless wide-field microscopy imaging platform based on digital inline holography and multi-frame pixel...

Area of Science:

• Microscopy
• Optical Imaging
• Computational Imaging

Background:

• Traditional microscopy faces limitations in resolution and field-of-view for biological samples.
• Lensless imaging offers a compact alternative but often requires advanced computational techniques for high resolution.

Purpose of the Study:

• To develop an irregular displacement-based lensless wide-field microscopy platform.
• To enhance spatial resolution using computational pixel super-resolution with multi-frame processing.

Main Methods:

• Combining digital in-line holography with a multi-frame pixel super-resolution approach.
• Utilizing a hybrid method for displacement resolution: feature-based registration and continuous optimization.
• Applying numerical phase-retrieval to reconstruct sample morphology.

Main Results:

• Achieved a spatial resolution of 1.55 µm.
• Demonstrated imaging capability on biological samples like sperm and platelets (micron-scale).
• Operated on a wide field-of-view of approximately 30 mm².

Conclusions:

• The presented lensless microscopy platform effectively combines digital holography and super-resolution for enhanced biological imaging.
• The multi-frame processing and hybrid displacement resolution method significantly improve spatial resolution.
• This technique offers a promising approach for high-resolution, wide-field imaging of microscopic biological structures.