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Metabolomic study of human tissue and urine in clear cell renal carcinoma by LC-HRMS and PLS-DA

Joanna Nizioł1, Vincent Bonifay2, Krzysztof Ossoliński3

  • 1Faculty of Chemistry, Rzeszow University of Technology, 35-959, Rzeszow, Poland. jniziol@prz.edu.pl.

Analytical and Bioanalytical Chemistry
|April 17, 2018
PubMed

Insights

Researchers identified potential biomarkers for clear cell renal cell carcinoma (ccRCC) by analyzing kidney tissue and urine. Elevated levels of specific carnitines and norepinephrine sulfate in cancer patients may aid in early detection of this kidney cancer.

Area of Science:

  • Metabolomics
  • Biomarker Discovery
  • Urology
  • Oncology

Background:

  • Renal cell carcinoma (RCC) is a significant cause of cancer-related mortality.
  • Current clinical management of kidney cancer lacks validated tissue biomarkers.
  • The need for reliable biomarkers for early diagnosis and treatment monitoring of ccRCC is critical.

Purpose of the Study:

  • To identify potential urinary and tissue biomarkers for clear cell renal cell carcinoma (ccRCC).
  • To investigate metabolic differences between cancerous and normal kidney tissues and associated urine samples.
  • To discover novel up- and downregulated compounds indicative of renal cancer.

Main Methods:

  • Non-targeted metabolomic analysis of paired surgically removed renal cancer and normal tissue.
  • Analysis of urine samples from ccRCC patients and healthy controls.
  • Liquid chromatography/high-resolution mass spectrometry (LC-HRMS) for compound identification.

Main Results:

  • Hydroxybutyrylcarnitine, decanoylcarnitine, propanoylcarnitine, carnitine, dodecanoylcarnitine, and norepinephrine sulfate were significantly elevated in ccRCC tissues and patient urine.
  • Riboflavin and N-acetylaspartylglutamate (NAAG) were found at higher concentrations in normal kidney tissue and healthy urine.
  • These identified compounds show potential as diagnostic biomarkers for ccRCC.

Conclusions:

  • The study successfully identified several candidate biomarkers for ccRCC.
  • Metabolomic profiling of tissue and urine offers a promising approach for kidney cancer biomarker discovery.
  • Further validation is required to establish these compounds as reliable clinical biomarkers for renal cell carcinoma.
Keywords:
BiomarkerCancer biomarkerKidneyMass spectrometryRenal cell carcinoma

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