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Identification of serotoninergic system components in stallion sperm
F Jiménez-Trejo1, I Coronado-Mares2, M Boeta3
1National Institute of Pediatrics, Coyoacán, Mexico City, Mexico. trejofj@hotmail.com.
Histology and Histopathology
|April 17, 2018
Summary
Serotonin (5-HT) markers were found in stallion sperm, suggesting its potential as a pharmacological target to improve conception rates in horses through enhanced sperm function.
Area of Science:
- Reproductive Biology
- Animal Science
- Neuroendocrinology
Background:
- Assisted reproductive techniques in domestic animals have low efficiency in equines.
- Pharmacological treatments are explored to increase stallion fertility by promoting sperm hyperactivation.
- Previous research suggests serotonin (5-HT) may enhance conception rates in horses.
Purpose of the Study:
- To investigate the presence and function of the serotoninergic system in stallion sperm.
- To identify specific serotonin receptors, enzymes, and transporters in equine sperm.
- To explore the potential of targeting the serotoninergic system for improving equine fertility.
Main Methods:
- Immunofluorescence and western blot techniques were employed.
- Stallion sperm were analyzed for serotonin (5-HT) and related markers.
- Detection of 5-HT receptors (5-HT1B, 5-HT2A, 5-HT3), enzymes (TPH1, MAOA), transporters (5-HTT, V-MAT1) was performed.
Main Results:
- Serotonin markers (5-HT, 5-HT1B, 5-HT2A, 5-HT3, TPH1, MAOA, 5-HTT) were identified in stallion sperm.
- A novel V-MAT1 transporter was detected in stallion sperm, not previously reported in mammalian sperm.
- The findings indicate the presence of a functional serotoninergic system within stallion sperm.
Conclusions:
- The serotoninergic system is present in stallion sperm.
- This system represents a potential pharmacological target for enhancing male fertility in horses.
- Targeting serotonin pathways could improve conception rates in domestic horses.
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