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Updated: Feb 11, 2026

In Vivo Microinjection and Electroporation of Mouse Testis
Published on: August 23, 2014
[Expression characteristics of the Daxx gene in the mouse testis during spermatogenesis]
Zeng Zhang1, Qiong Deng2, Yong Wu2
1Graduate School, Anhui Medical University, Hefei, Anhui 230032, China.
Objective:
To investigate the expression characteristic of the Daxx gene in the mouse testis and its role in spermatogenesis.
Methods:
Real-time PCR, Western blot and immunofluorescence were used in examining the expression characteristics of DAXX in the testis tissue from wild-type, Sertoli cell-specific androgen receptor knockout (SCARKO) and androgen receptor knockout (ARKO) mice at different postnatal weeks .
Results:
The Daxx gene was highly expressed in the testis tissue and mainly in the nuclei of the wild-type mice at 4 postnatal weeks. Compared with the wild-type, the ARKO mice showed a markedly decreased expression of DAXX (0.299±0.026), which displayed a polar distribution in the spermatogenic cells (0.853±0.058) and exhibited no significant difference in the SCARKO mice (1.000±0.015).
Conclusions:
The Daxx gene expression is the highest in the middle-stage development of the mouse testis, significantly decreased in ARKO mice as compared with the wild-type, and its location influenced by specific AR knockout in Sertoli cells. DAXX may be involved in the regulation of spermatogenesis in mice.
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