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Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
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Development and application of CRISPR/Cas9 technologies in genomic editing
Cui Zhang1, Renfu Quan2, Jinfu Wang1
1Institute of Cell and Development Biology, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang, P.R. China.
Human Molecular Genetics
|April 17, 2018
Summary
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system offers precise genome editing for treating diseases. Recent advancements have expanded its use in base editing, gene regulation, and therapeutic applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Genomic editing holds promise for treating human diseases by correcting mutations.
- The CRISPR/Cas9 system is a powerful, programmable tool for precise genome modification.
Purpose of the Study:
- To review recent developments and applications of CRISPR/Cas9 technology over the past two years.
- To discuss the future prospects and challenges of CRISPR/Cas9 in medicine.
Main Methods:
- Literature review of CRISPR/Cas9 applications.
- Analysis of advancements in base editing, transcription modulation, epigenetic editing, and genomic screening.
- Examination of CRISPR/Cas9 use in cell and embryo therapy.
Main Results:
- CRISPR/Cas9 technology has seen rapid development, broadening its applications.
- The system is being applied in base editing, transcriptional modulation, epigenetic editing, and large-scale genomic screening.
- CRISPR/Cas9 is advancing in preclinical trials for cell and embryo therapies.
Conclusions:
- CRISPR/Cas9 technology is rapidly evolving with diverse applications in gene therapy and research.
- Continued development and ethical considerations are crucial for the clinical translation of CRISPR/Cas9 therapies.
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