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Updated: Feb 11, 2026
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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
Published on: November 18, 2011
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Vitrification tendency and stability of DP6-based vitrification solutions for complex tissue cryopreservation
Brian Wowk1, Gregory M Fahy1, Susan Ahmedyar1
121st Century Medicine, Inc., Fontana, CA 92336, United States.
Cryobiology
|April 17, 2018
Summary
Vitrification solutions like DP6 were enhanced with additives to improve cryoprotection. Adding 1,3-cyclohexanediol significantly reduced the critical cooling rate, enhancing ice suppression for better vitrification.
Area of Science:
- Cryobiology and Materials Science
- Thermophysical properties of cryoprotective agents
Background:
- Vitrification solutions are crucial for cryopreservation, preventing damaging ice crystal formation.
- DP6 is a vitrification solution whose properties are being characterized for cryoprotective applications.
- Enhancing ice suppression in vitrification solutions is key to improving cryopreservation outcomes.
Purpose of the Study:
- To analyze the vitrification tendency and amorphous state stability of DP6.
- To evaluate the effectiveness of various solutes in enhancing ice suppression for DP6.
- To characterize the thermophysical properties, including critical cooling and warming rates, of DP6 and its modified formulations.
Main Methods:
- Differential scanning calorimetry (DSC) was employed to analyze vitrification and devitrification.
- The critical cooling rate (CCR) for vitrification was determined for DP6 and its derivatives.
- Critical warming rates (CWR) to avoid devitrification were measured for all tested solutions.
Main Results:
- DP6 exhibited a critical cooling rate of 2.7 °C/min.
- Additives like 1,3-cyclohexanediol and PEG 400 significantly reduced the CCR to below 1 °C/min.
- Critical warming rates were substantially lowered by additives, with 1,3-cyclohexanediol and PEG 400 showing the most significant ice suppression.
Conclusions:
- Additives effectively enhance the ice suppression capabilities of DP6, reducing critical cooling and warming rates.
- 1,3-cyclohexanediol demonstrated the highest efficacy in suppressing ice formation on a per-mass basis.
- The modified DP6 solutions show improved potential as cryoprotective agents due to enhanced vitrification stability.
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