Related Experiment Video
Updated: Feb 11, 2026
14:53
High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
Published on: May 19, 2011
18.5K
AIE-based superwettable microchips for evaporation and aggregation induced fluorescence enhancement biosensing
Yanxia Chen1, Xuehong Min2, Xiqi Zhang3
1Research Center for Bioengineering and Sensing Technology, Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
Biosensors & Bioelectronics
|April 17, 2018
Summary
This study introduces an aggregation-induced emission (AIE) superwettable microchip for sensitive fluorescence detection. The novel platform enhances fluorescence signals for improved diagnostics and biomolecule analysis.
Area of Science:
- Biomedical diagnostics
- Materials science
- Analytical chemistry
Background:
- Superwettable microchips are crucial for fluorescence-based diagnostics.
- Traditional fluorophores face aggregation-caused quenching (ACQ) issues at high concentrations.
Purpose of the Study:
- To develop an aggregation-induced emission (AIE) based superwettable microchip.
- To combine evaporation-induced enrichment with AIE for enhanced fluorescence detection.
Main Methods:
- Developed an AIE-based superwettable microchip integrating evaporation-induced enrichment and AIE properties.
- Utilized tetraphenylethene salt (TPE-Z) as the AIE molecule.
- Detected microRNA-141 (miR-141) using the developed microchip.
Main Results:
- Achieved significant fluorescence enhancement due to synergistic effects of evaporation and AIE.
- Demonstrated high reproducibility, sensitivity, and specificity in miR-141 detection.
- Obtained a low detection limit of 1 pM with an improved signal-to-noise ratio compared to traditional probes.
Conclusions:
- The AIE-based superwettable microchip offers a simple, effective platform for fluorescence enhancement.
- This technology enables rapid, multiplexed, and high-throughput analysis for various applications.
- Potential applications include environmental monitoring, food safety, and medical diagnostics.
Related Concept Videos
03:00Self-Evaluation: Self-Enhancement and Self-Verification
5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
01:29Aggregates Classification
1.1K
Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
Petrographic classification groups aggregates based on common mineralogical characteristics. Some of the common mineral groups found in aggregates are...
Petrographic classification groups aggregates based on common mineralogical characteristics. Some of the common mineral groups found in aggregates are...
1.1K
01:16Bioavailability Enhancement: Drug Solubility Enhancement
273
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
273
01:27Bioavailability Enhancement: Drug Permeability Enhancement
213
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
213
01:12Bonding and Strength of Aggregate
519
The bond between aggregate particles and the cement matrix is significantly influenced by the shape and surface texture of the aggregates. High-strength concretes benefit from a rougher texture, which leads to stronger bonding due to greater adhesion. Angular aggregates with larger surface areas also enhance this bond. The bonding quality, however, is complex to assess as no universally accepted test exists. Good bonding is indicated when a crushed concrete specimen shows some aggregate...
519
01:19Specific Gravity of Aggregate
845
Aggregates typically contain pores, which can be either permeable or impermeable. Considering the pores in the aggregates, the specific gravity of aggregates is defined in three different forms, namely, bulk or gross specific gravity, apparent specific gravity, and absolute specific gravity.
Bulk or gross specific gravity is calculated by taking the ratio of the mass of aggregates in the saturated surface-dry state to the total volume that includes both the solids and the voids within the...
Bulk or gross specific gravity is calculated by taking the ratio of the mass of aggregates in the saturated surface-dry state to the total volume that includes both the solids and the voids within the...
845