Related Experiment Video
Updated: Feb 11, 2026
07:41
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
Published on: March 8, 2022
2.9K
Development of a cost effective and robust AlphaScreen® platform for application
John R Veloria1, Ashwini K Devkota1, Eun Jeong Cho1
1Targeted Therapeutic Drug Discovery & Development Program, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA.
Biotechniques
|April 18, 2018
Summary
This study optimizes AlphaScreen detection by reducing reagent costs through lower bead concentrations. The new protocol maintains assay integrity, making high-throughput screening more accessible and affordable.
Area of Science:
- Biochemistry
- Assay Development
- High-Throughput Screening
Background:
- AlphaScreen detection is valuable for molecular interaction studies.
- High reagent costs limit AlphaScreen use in extensive screening and for researchers with limited funding.
- Current cost-reduction strategies primarily focus on miniaturization, not reagent scaling.
Purpose of the Study:
- To optimize the AlphaScreen detection platform by reducing reagent concentrations.
- To decrease assay costs without compromising assay integrity.
- To enhance the accessibility and utilization of AlphaScreen technology.
Main Methods:
- Systematically reduced AlphaScreen bead concentrations.
- Optimized the detection platform down to 2.5 μg/ml beads.
- Evaluated assay integrity and statistical robustness at reduced concentrations.
Main Results:
- Successfully reduced AlphaScreen bead concentration to 2.5 μg/ml.
- Demonstrated substantial cost reduction in detection.
- Maintained robust statistics and assay integrity.
- Achieved significant cost savings without compromising assay performance.
Conclusions:
- Reduced bead concentration is a viable strategy for lowering AlphaScreen assay costs.
- The optimized protocol enhances the economic feasibility of AlphaScreen for high-throughput screening.
- This approach will likely increase the adoption of AlphaScreen technology by researchers.
Related Concept Videos
02:19Buffer Effectiveness
55.6K
Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
The buffer capacity is the amount of acid or base that can be added to a given volume...
55.6K
03:26Framing Effects
8.0K
Information is everywhere and its presentation—such as how and when items are presented—can impact our perceptions and decisions surrounding the info. This broad concept umbrellas framing effects—influences that occur due to the way information is framed in its appearance, whether it’s purely the order or the specific wording of a message. Let’s take a look at numerous ways in which two versions of something can objectively say the same thing, yet we respond in...
8.0K
02:59Biological Effects of Radiation
18.1K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
18.1K
01:43Sustainable Development
15.2K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
15.2K
01:21Self-Awareness and Its Effects
318
Self-awareness is a psychological state in which the individual becomes the focal point of their attention. This inward focus transforms the self into an object of contemplation and assessment, influencing how individuals perceive their actions and their alignment with personal and societal standards.Triggers and Contexts for Self-AwarenessSelf-awareness can be activated by external stimuli that make individuals visually or audibly aware of themselves, such as mirrors, cameras, or recordings.
318
01:24Effects of feedback
1.1K
Feedback in control systems plays a critical role in shaping various operational parameters, extending beyond simple error reduction to influence stability, bandwidth, gain, impedance, and sensitivity. Understanding these effects requires examining a basic feedback system characterized by defined input, output, error, and feedback signals.
Feedback significantly modifies the gain of a control system. The gain of a system without feedback is altered by a factor of one plus GH, where G represents...
Feedback significantly modifies the gain of a control system. The gain of a system without feedback is altered by a factor of one plus GH, where G represents...
1.1K