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Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
Published on: September 28, 2022
Different methods of methyl eugenol application enhance the mating success of male Oriental fruit fly (Dipera:
Ihsan Ul Haq1,2, Carlos Cáceres3, José S Meza3
1Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Seibersdorf, Austria. imihsan@yahoo.com.
Abstract:
Males of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) are strongly attracted to methyl eugenol (ME) (1,2-dimethoxy-4-(2-propenyl)benzene), a phenylpropanoid compound occurring in many plant species. Feeding on ME is known to enhance male B. dorsalis mating competitiveness, which can increase the effectiveness of the sterile insect technique (SIT) manifold. However, currently used systems for holding the mass-reared males in fly emergence and release facilities before release, do not allow for application of ME through feeding. Therefore, the current study was designed to evaluate different delivery systems of ME that would be applicable for large-scale application to sterile males held in such facilities. Males of a genetic sexing strain (GSS) of B. dorsalis treated by ME-aromatherapy or ME-airblown-aromatherapy that were competing with ME-fed males achieved a similar level of mating success in walk-in field cages, but the mating success was significantly higher when compared to untreated males. The results confirm the feasibility of developing ME-airblown-aromatherapy as a practical way of large scale ME delivery to enhance the mating competitiveness of sterile B. dorsalis males.
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Key Attributes include the following:
Mate Choice
Infertility in Males
Location and Orientation of the Heart

