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Related Experiment Video

Updated: Feb 11, 2026

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes
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Comparative study of Sperm Motility Analysis System and conventional microscopic semen analysis.

Kazuhiko Komori1, Akira Tsujimura1, Sumio Ishijima2

  • 1Department of Urology, Osaka University Graduate School of Medicine, Suita, Osaka and.

Reproductive Medicine and Biology
|April 18, 2018
PubMed
Summary

A new Sperm Motility Analysis System (SMAS) shows strong correlation with manual semen analysis, offering a promising alternative for accurate sperm analysis. This automated system enhances consistency in evaluating sperm concentration and motility.

Keywords:
Sperm Motility Analysis Systemsemen analysis

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Area of Science:

  • Reproductive Medicine
  • Andrology
  • Biotechnology

Background:

  • Conventional manual sperm analysis exhibits variability despite World Health Organization (WHO) guidelines.
  • Standardized methods are crucial for reliable semen analysis in clinical and research settings.

Purpose of the Study:

  • To compare the accuracy and reliability of a new Sperm Motility Analysis System (SMAS) against established manual semen analysis methods based on WHO guidelines.
  • To evaluate SMAS for its potential to reduce variability in sperm concentration and motility assessments.

Main Methods:

  • Simultaneous analysis of semen samples from 30 infertility patients and 21 healthy volunteers using both manual microscopy (WHO guidelines) and the SMAS.
  • Comparative statistical analysis of sperm concentration and percent motility obtained from both methods.

Main Results:

  • Strong positive correlation observed between SMAS and manual analysis for sperm concentration (r=0.95, P<0.001) and percent motility (r=0.89, P<0.001).
  • Excluding high sperm concentration samples (>30 × 10^6/mL) further improved the correlation for sperm concentration (r=0.81, P<0.001).

Conclusions:

  • The Sperm Motility Analysis System (SMAS) demonstrates high correlation with WHO-based manual sperm analysis.
  • SMAS presents a reliable and promising automated alternative for objective semen analysis, potentially improving diagnostic consistency.