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The interferon gamma receptor.

A Celada1

  • 1Cancer Research Center, La Jolla Cancer Research Foundation, CA 92037.

Lymphokine Research
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Interferon-gamma (IFN gamma) binds to cell surface receptors to regulate cell functions. Different cellular responses require varying levels of receptor occupancy, indicating distinct activation pathways.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Interferon-gamma (IFN gamma) modulates diverse cellular functions.
  • IFN gamma signaling involves specific cell surface receptors.
  • Species specificity in IFN gamma action is linked to ligand-receptor interactions.

Purpose of the Study:

  • To investigate the mechanisms of IFN gamma receptor interaction and cellular response.
  • To determine the relationship between receptor occupancy and biological outcomes.
  • To elucidate how IFN gamma triggers different cellular activation pathways.

Main Methods:

  • Detection of IFN gamma receptors in human and murine cell types.
  • Analysis of IFN gamma internalization and degradation rates.

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  • Comparison of receptor occupancy levels with the induction of specific cellular functions in macrophages.

    • Main Results:

    • IFN gamma receptors are present on various human and murine cells.
    • Human IFN gamma receptor is a 90 kd protein; receptor recycling and intracellular pools facilitate continuous uptake.
    • Simple functions (H2O2 secretion, Fc receptor expression) require one receptor occupancy round, while complex functions (tumoricidal activity) require multiple rounds.

    Conclusions:

    • IFN gamma induces distinct cellular activities through differential activation pathways.
    • The number of receptor occupancy rounds correlates with the complexity of the induced cellular function.
    • Cell surface and intracellular events are critical for mediating the diverse effects of IFN gamma.